What is an HP LC test? Many friends who come to me to buy plant extracts have asked about the test method. At present, there are many detection methods used. The most common one is H PLC. Let me introduce it to you
In 1903, Russian plant chemist Tswett first proposed the concept of “Chromatography” and “Chromatogram”. Zvett described his color experiments by means of chromatography (from the Greek word Chroma, meaning color, and graphy, meaning to record — literally, to record color). (Curiously, the Russian name Zvett means color.) In his paper, he wrote:
“(sic) a petroleum ether solution of plant pigment was added from the top of a glass tube containing mainly calcium carbonate adsorbent, filtered down the tube, and then eluted with pure petroleum ether. As a result, the corresponding bands of different pigments were observed in the tube according to the order of adsorption, just like the spectrum, which was called chromatograms.”
After 1930, liquid chromatography techniques such as paper chromatography, ion exchange chromatography and thin layer chromatography appeared successively.
In 1952, British scholars Martin and Synge put forward a relatively complete theory and method on gas-liquid distribution chromatography based on their research work in distribution chromatography, which made chromatography technology a big step forward. This is the reason for the rapid development of gas chromatography in the following ten years.
In 1958, based on the work of Moore and Stein, the instrumentalization of ion-exchange chromatography led to the development of the amino acid analyzer, which is an important attempt of modern liquid chromatography, but the separation efficiency is still not ideal.
In the middle and late 1960, the development of gas chromatography theory and practice, as well as mechanical, optical, electronic and other technical progress, liquid chromatography began to be active. The use of high pressure pumps and chemically bonded stationary phases for liquid chromatography came into being in the late 1960s.
Since the mid-1970s, microprocessor technology has been used in liquid chromatography, further improving the level of automation and analytical accuracy of the instrument.
After 1990, the rapid development of bioengineering and life science in the international and domestic, for high performance liquid chromatography technology put forward more, updated separation, purification, preparation of the subject, such as the human genome project, proteomics with HPLC for pre-separation, etc..